The effects of medium composition on biomass, sterols and triterpenols production by in-vitro cultures of Euphorbia characias

• Type: Article in International Scientific Journal
• Date: 1992

Authors

FERNANDESFERREIRA, M (Author) Other; PAIS, MSS (Author) Other; NOVAIS, JM (Author) Other

Journal

Title: Bioresource Technology

Vol. 42

Pages: 67-73

ISSN: 0960-8524

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 3 Citations

Scopus - 4 Citations

Scientific classification

FOS: Agrarian Sciences > Agricultural biotechnology

Other information

Authenticus ID: P-00H-70S

DOI: 10.1016/0960-8524(92)90089-g

Abstract (EN): Calli of Euphorbia characias L. maintained on Murashige-Skoog (MS) medium (1962, Physiol. Plant., 15, 473-97) were grown on 20 other basal media. The highest callus biomass production was obtained on half-strength MS and the lowest on Cambell-Durzan medium (1975, Can. J. Bot., 53, 1652-6). Independently of the culture medium used, calli produced campesterol, beta-sitosterol, DELTA-5-avenasterol and alpha-amyrin. The Gautheret medium (1942, Manuel Technique de Culture des Tissus Vegetaux, Masson Cie, Paris) was the most effective for the production of sterols and alpha-amyrin. The increase in sucrose supplementation increased callus biomass production but decreased the specific sterols and triterpenols content/dry weight. The increase in sucrose increased the specific phenolics content. The specific sterols and triterpenols content of in-vitro cultures increased during the exponential phase of biomass growth.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7