Automatic Multi-pumping Flow System for the Chemiluminometric Screening of Scavenging Capacity against Singlet Oxygen

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Ana F T Silva (Author) Other; Karine L Marques (Author) Other; Joao L M Santos (Author) FFUP; Jose L F C Lima (Author) FFUP

Journal

Title: Analytical Sciences

Vol. 27 No. 5

Pages: 827-832

ISSN: 0910-6340

Publisher: Japan Society for Analytical Chemistry

Indexing

ISI Web of Knowledge - 2 Citations

ISI Web of Science

Scopus - 3 Citations

Pubmed / Medline

Scientific classification

FOS: Natural sciences > Chemical sciences

CORDIS: Physical sciences > Chemistry > Analytical chemistry

Other information

Authenticus ID: P-002-P44

DOI: 10.2116/analsci.27.827

Resumo (PT): An automated multi-pumping flow system was developed for the in-line generation of singlet oxygen (1O2) and subsequent assessment of the scavenging capacity against this reactive species. 1O2 was generated by dismutation of hydrogen peroxide catalyzed by molybdate ions. The evaluation of the scavenging capacity was based on the inhibition of the chemiluminescence reaction of luminol with 1O2. The proposed system used solenoid micro-pumps as the only active components of the flow manifold, enabling the reproducible insertion and efficient mixing of sample and reagents as well as the transportation of the sample zone towards detection for chemiluminescence measurement, assuring a strictly reproducible timing of all analytical tasks. Several compounds were evaluated as possible 1O2 scavengers. The obtained results showed that only ascorbic acid, dipyrone and tryptophan exhibited scavenging capacity, with IC50 values of 3.36 × 10−5, 7.84 × 10−5 and 1.28 × 10−2 mol L−1, respectively <br> <br> <a target="_blank" href="http://www.jstage.jst.go.jp/article/analsci/27/8/27_827/_article "> Texto integral </a> <br>

Abstract (EN): An automated multi-pumping flow system was developed for the in-line generation of singlet oxygen (O-1(2)) and subsequent assessment of the scavenging capacity against this reactive species. O-1(2) was generated by dismutation of hydrogen peroxide catalyzed by molybdate ions. The evaluation of the scavenging capacity was based on the inhibition of the chemiluminescence reaction of luminol with O-1(2). The proposed system used solenoid micro-pumps as the only active components of the flow manifold, enabling the reproducible insertion and efficient mixing of sample and reagents as well as the transportation of the sample zone towards detection for chemiluminescence measurement, assuring a strictly reproducible timing of all analytical tasks. Several compounds were evaluated as possible O-1(2) scavengers. The obtained results showed that only ascorbic acid, dipyrone and tryptophan exhibited scavenging capacity, with IC50 values of 3.36 x 10(-5), 7.84 x 10(-5) and 1.28 x 10(-2) mol L-1, respectively.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6