Significant Differences in the Reversal of Cellular Stress Induced by Hydrogen Peroxide and Corticosterone by the Application of Mirtazapine or L-Tryptophan

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Correia, AS (Author) Other; Armando Cardoso (Author) FMUP; Nuno Vale (Author) FMUP

Journal

The Journal is awaiting validation by the Administrative Services.

Title: International Journal of Translational Medicine

Vol. 2

Pages: 482-505

ISSN: 2673-8937

Other information

Authenticus ID: P-00X-49G

DOI: 10.3390/ijtm2030036

Abstract (EN): <jats:p>Depression is a prevalent and debilitating disease worldwide. This pathology is very complex and the lack of efficient therapeutic modalities, as well as the high rates of relapse, makes the study and treatment of depression a global healthcare challenge. Thus, an intense investigation of this disease is crucial and urgent. In this study, we focused on hydrogen peroxide and corticosterone-induced stress on SH-SY5Y and HT-22 cells. Additionally, we aimed to study the potential attenuation of these induced stress with the exposure of both cells to mirtazapine and L-tryptophan, focusing on cell viability assays (MTT and Neutral Red) and reactive oxygen species production assays (DCFDA fluorescence). Taken together, our results indicate that mirtazapine and L-tryptophan counteract the cellular stress induced by hydrogen peroxide but not by corticosterone, revealing a potential role of these agents on oxidative stress relief, highlighting the role of serotonergic pathways in the oxidative stress present in depressed individuals. This study allows the investigation of depression using cellular models, enabling the screening of compounds that may have potential to be used in the treatment of depression by acting on cellular mechanisms such as oxidative stress protection.</jats:p>

Language: English

Type (Professor's evaluation): Scientific