Prolonged exposure to bradykinin and prostaglandin E2 increases TRPV1 mRNA but does not alter TRPV1 and TRPV1b protein expression in cultured rat primary sensory neurons

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Mistry, S (Author) Other; Paule, CC (Author) Other; Varga, A (Author) Other; Photiou, A (Author) Other; Jenes, A (Author) Other; António Avelino Silva (Author) FMUP; Buluwela, L (Author) Other; Nagy, I (Author) Other

Journal

Title: Neuroscience Letters

Vol. 564

Pages: 89-93

ISSN: 0304-3940

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 10 Citations

Scopus - 11 Citations

Other information

Authenticus ID: P-009-B3C

DOI: 10.1016/j.neulet.2014.02.006

Abstract (EN): Sensitisation of the capsaicin receptor, transient receptor potential vanilloid type 1 (TRPV1) ion channel in nociceptive primary sensory neurons (PSN) underlies the development of inflammatory heat hyperalgesia. Removal of the negative-dominant splice variant of the TRPV1 molecule, TRPV1b from TRPV1/TRPV1b heterotetrameric channels, which should be associated with changes in the expression of TRPV1 and TRPV1b transcripts and proteins, has been suggested to contribute to that sensitisation. Respective reverse-transcriptase polymerase chain reaction (RT-PCR) and Western-blotting revealed that both TRPV1 and TRPV1b mRNA, and their encoded proteins are expressed in rat cultured PSN. Sequencing of the RT-PCR products showed that TRPV1b mRNA lacks the entire exon 7. Further, growing PSN for 2 days in the presence of 10 mu M bradykinin (BK) and 10 mu M prostaglandin E-2 (PGE(2)) significantly increases TRPV1 responsiveness and TRPV1 mRNA expression, without producing any changes in TRPV1b mRNA, and TRPV1 and TRPV1b protein expression. These data challenge the hypothesis that alterations in the composition of the TRPV1 ion channel contributes to the sensitisation.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5