Ofloxacin Determination in Urine, Serum and Pharmaceuticals Using an Automatic Flow Potentiometric System

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Pimenta, AM (Author) Other; Souto, MRS (Author) Other; Catarino, RIL (Author) Other; Leal, MFC (Author) Other; Lima, JLFC (Author) FFUP

Journal

Title: Analytical Sciences

Vol. 29

Pages: 893-898

ISSN: 0910-6340

Publisher: Japan Society for Analytical Chemistry

Indexing

ISI Web of Knowledge - 3 Citations

Scopus - 2 Citations

Other information

Authenticus ID: P-008-ENK

DOI: 10.2116/analsci.29.893

Abstract (EN): An automatic system was developed to determine ofloxacin in biological fluids and pharmaceutical formulations. Drug detection was carried out by a potentiometric membrane sensor based on [bis(trifluoromethyl)phenyl]borate as molecular-recognition material. The tubular shaped detector system was solidly attached to the manifold, creating a high-throughput stable setup (50 samples per hour) appropriate for routine antibiotic assessment. Under the optimized flow conditions, the sensor displayed a mean detection limit of 1 x 10(-5) M, a linear response over the concentrations of 2 x 10(-5) to 5 x 10(-3) M (slope of 57.4 mV decade(-1)) and a wide working pH range (2.1 - 6.6). The procedure was successfully applied to ofloxacin analysis in pharmaceuticals (relative deviation lower than 6%) and biological fluids at levels usually found after drug administration of clinical doses (recoveries between 91 and 106%). No significant interference from common excipients found in commercial formulations and inorganic ions usually present in biological fluids was noticed.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6