Enzymic flow injection determination of free L-carnitine in infant formulae

• Type: Article in International Scientific Journal
• Date: 1997

Authors

Ferreira, IMPLVO (Author) FFUP; Macedo, MN (Author) Other; Ferreira, MA (Author) Other

Journal

Title: The Analyst

Vol. 122

Pages: 1539-1541

ISSN: 0003-2654

Publisher: Royal Society of Chemistry

Indexing

ISI Web of Knowledge - 14 Citations

Scopus - 14 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-001-A5H

DOI: 10.1039/a704903j

Abstract (EN): Infant formula samples mere analysed to determine their free L-carnitine content by using flow injection (FI) with an incorporated immobilised carnitine acetyltransferase bioreactor. The methodology was based on the spectrophotometric determination through its reaction with carnitine acetyltransferase coupled with acetyl coenzyme A (acetyl-CoA) and dithiobenzoate. The merging zones technique was used to minimise acetyl CoA consumption. Linearity was observed over the concentration range 10-80 mg l(-1) with L-carnitine as standard (r = 0.9998) and the rate of analysis was 50 h(-1) infant formula samples. The enzymic FI method afforded a low RSD (2.2%). Comparisons were made with other methods of known accuracy. The enzymic reactors were stable for 3 months when used daily at the optimum pH.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 3