Separation Processes in Biotechnology

Code:

EQ0110

Acronym:

PSB

Keywords
Classification	Keyword
OFICIAL	Natural Sciences (Biological Sciences)

Instance: 2011/2012 - 1S

Active?	Yes
Responsible unit:	Department of Chemical Engineering
Course/CS Responsible:	Master in Chemical Engineering

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
MIEQ	11	Syllabus	4	-	5	56	135
			5

Teaching language

Portuguese

Objectives

This course aims to acquaint students with underlying knowledge on engineering regarding purification processes of biological products, accreditation and regulation of these products.
This course also aims to develop students’ reasoning and capacity of analysing product flowsheets and to use bioinformatic tools.

Program

1- Biotechnology and bio products

2- Introduction to purification of biological products

3- Cellular rupture
a) Mechanical methods
b) Non-mechanical methods

4- Solid-liquid Separations
a) Sedimentation
b) Centrifugation
c) Conventional filtration
d) Membrane processes

5- Chromatography
a) Gel filtration
b) Ion exchange
c) Affinity
d) Hydrophobic interaction

6- Extraction processes
7- Precipitation and crystallization

8- Solvent removal
a) Drying
b) Freeze-drying
c) Evaporation

9- Product activity

10- Regulation
a) Validation
b) Approval
c) Formulation

11-Bioinformatic analysis of proteins

12- Analysis of product flowsheets

13- Simulation of purification processes

Mandatory literature

Harrison, Roger G. 070; Bioseparations science and engineering. ISBN: 0-19-512340-9
Filipe Mergulhão ; Textos de apoio da disciplina (A fornecer aos alunos em formato electrónico), 2009

Complementary Bibliography

Crommelin, Daan J. A. 340; Pharmaceutical biotechnology. ISBN: 0-415-28501-1
Wink, M. (ed); An introduction to molecular biotechnology, Wiley-VCH, 2006. ISBN: 3-527-31412-1

Teaching methods and learning activities

Classes will be based on the oral presentation of the themes of the course with support of the black board and slides. In some classes the computer will be used in order to use simulation software and bioinformatic tools available on the Internet.

Software

Office 2000

keywords

Natural sciences > Biological sciences > Biological engineering
Technological sciences > Engineering

Evaluation Type

Distributed evaluation without final exam

Eligibility for exams

Students have to attend classes to be admitted to exams- according to General Evaluation Rules of FEUP

Calculation formula of final grade

The final grade will be calculated by a weighted average between all the components of a group project assignment and the grades obtained on the oral presentations and project log book. The weights of each component will be discussed on class.

Examinations or Special Assignments

Students have to do a group assignment, which has to be done during the semester (some parts can be done in classes):

The group assignment comprises:
a) Introduction
b) Patent research
c) Diagrams
d) Bioinformatic analysis of proteins
e) Simulation of purification processes
f) Conclusion

Special assessment (TE, DA, ...)

An exam at resit period - according to General Evaluation Rules of FEUP

Classification improvement

Exam in the resit period