Improving aptamer performance with nucleic acid mimics: de novo and post-SELEX approaches

• Tipo: Outra Publicação em Revista Científica Internacional
• Data: 2022

Autores

Oliveira, R (Autor) Outra; Pinho, E (Autor) Outra; Sousa, AL (Autor) Outra; DeStefano, JJ (Autor) Outra; Azevedo, NF (Autor) FEUP; Almeida, C (Autor) Outra

Revista

Título: Trends in Biotechnology

Vol. 40

Páginas: 549-563

ISSN: 0167-7799

Editora: Elsevier

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 0 Citações

Outras Informações

ID Authenticus: P-00V-MJ5

DOI: 10.1016/j.tibtech.2021.09.011

Abstract (EN): Aptamers are structural single-stranded oligonucleotides generated in vitro to bind to a specific target molecule. Aptamers' versatility can be enhanced with nucleic acid mimics (NAMs) during or after a selection process, also known as systematic evolution of ligands by exponential enrichment (SELEX). We address advantages and limitations of the technologies used to generate NAM aptamers, especially the applicability of existing engineered polymerases to replicate NAMs and methodologies to improve aptamers after SELEX. We also discuss the limitations of existing methods for sequencing NAM sequences and bioinformatic tools to predict NAM aptamer structures. As a conclusion, we suggest that NAM aptamers might successfully compete with molecular tools based on proteins such as antibodies for future application.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 15