Quantitative analysis of kielcorins in biomimetic synthesis by liquid chromatography/UV detection

• Type: Article in International Scientific Journal
• Date: 2003

Authors

Sousa, ME (Author) Other; Afonso, CMM (Author) FFUP; Pinto, MMM (Author) FFUP

Journal

Title: Journal of Liquid Chromatography and Related Technologies

Vol. 26

Pages: 29-41

ISSN: 1082-6076

Publisher: Taylor & Francis

Indexing

ISI Proceedings

ISI Web of Knowledge - 3 Citations

Scopus - 4 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-000-K16

DOI: 10.1081/jlc-120017150

Abstract (EN): An HPLC/UV assay was developed for separation and quantification of isomeric kielcorins in different synthetic crude products. trans(+/-)-Kielcorin C, cis-(+/-)-kielcorin C, trans-(+/-)-kielcorin D, trans-(+/-)isokielcorin D, and trans-(+/-)-kielcorin E were obtained by oxidative coupling of coniferyl alcohol and an appropriate xanthonic building block, 3,4-, 1,2-, and 2,3-dihydroxyxanthone, respectively, with silver carbonate, silver oxide, and potassium hexacyanoferrate (III). The isomeric kielcorins were separated and quantified directly in the nine crude products obtained. The assay was validated with respect to specificity, linearity, range, limits of quantification, and detection.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 13