Immobilization of laccase on modified silica: Stabilization, thermal inactivation and kinetic behaviour in 1-ethyl-3-methylimidazolium ethylsulfate ionic liquid

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Ana P. M. Tavares (Author) FEUP; Oscar Rodríguez (Author) FEUP; María Fernández-Fernández (Author) Other; Alberto Domínguez (Author) FEUP; Diego Moldes (Author) Other; María A. Sanromán (Author) Other; Eugénia A. Macedo (Author) FEUP

Journal

Title: Bioresource Technology

Vol. 131 No. March 2013

Pages: 405-412

ISSN: 0960-8524

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 43 Citations

ISI Web of Science

Scopus - 57 Citations

Scientific classification

FOS: Engineering and technology

CORDIS: Technological sciences

Associated Projects

LA 20 - PROJECTO ESTRATÉGICO - LA 20 - 2013-2014

E-27/09 - Ionic Liquids as Solvents for Enzymatic Catalysis, Acção Integrada Luso-Espanhola

Other information

Authenticus ID: P-005-2RJ

DOI: 10.1016/j.biortech.2012.12.139

Abstract (EN): Laccase was immobilized on modified silica carrier. The immobilization conditions, pH and enzyme concentration were optimized. Operational stability of 10 reaction cycles showed that immobilized laccase in buffer was stable, presenting an activity loss <30%. Nevertheless, a high decrease >80% was obtained in ionic liquid (IL) solution. Activity of immobilized laccase was maintained when incubated in IL After 7 days of incubation, immobilized laccase lost 30-50% of its initial activity. Immobilization also improved thermal stability of laccase in the presence of IL. Enzyme kinetics was modelled with Michaelis-Menten model. The K-m value for free laccase increases significantly with the IL concentration. Slight differences were found in V-m, for free enzyme. Unusual kinetic behaviour was obtained for immobilized laccase in IL: Both V-m and K-m increased with IL concentration, resulting in increased catalytic efficiency of the immobilized enzyme in presence of IL. (C) 2013 Published by Elsevier Ltd.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8