The effect of glucose concentration and shaking conditions on Escherichia coli biofilm formation in microtiter plates

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Joana M. R.Moreira (Author) FEUP; Luciana C. Gomes (Author) FEUP; José D. P. Araújo (Author) FEUP; João M. Miranda (Author) FEUP; Manuel Simões (Author) FEUP; Luís F. Melo (Author) FEUP; Filipe J. Mergulhão (Author) FEUP

Journal

Title: Chemical Engineering Science

Vol. 94 No. MAY 3 2013

Pages: 192-199

ISSN: 0009-2509

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 29 Citations

ISI Web of Science

Scopus - 36 Citations

INSPEC

Scientific classification

FOS: Engineering and technology > Chemical engineering

Associated Projects

SYNBIOBACTHER - Construção de bactérias "terapêuticas" usando uma abordagem de biologia sintética

Filmstart - Estudo das condições iniciais que controlam a formação de biofilmes

Other information

Authenticus ID: P-005-0T9

DOI: 10.1016/j.ces.2013.02.045

Abstract (EN): Microtiter plates are one of the most widely used platforms for biofilm studies. However, these systems have been used rather blindly without knowledge of the flow characteristics inside them. Plates with 96-wells were used to study flow dynamics and its influence on Escherichia coli JM109(DE3) biofilm formation. Two different glucose concentrations (1 g L-1 and 0.25 g L-1) and two different shaking conditions were tested using incubators with orbital diameters of 50 and 25 mm. Biofilm growth was monitored for 60 h and the hydrodynamics were simulated by computational fluid dynamics. These simulations have shown that wall shear stress was higher near the air/liquid interface and average values of 0.070 and 0.034 Pa were obtained for the largest and smallest orbital diameter. For the high glucose concentration, the maximum biofilm amount was attained at 24 h and similar values were obtained in both incubators. For the low glucose concentration, lower values were attained. Numerical simulations indicated that microtiter plates can adequately model biofilm formation in relevant biomedical systems.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8