Exploring Peracetic Acid and Acidic pH Tolerance of Antibiotic Resistant Non-typhoidal Salmonella and Enterococcus faecium from Diverse Epidemiological and Genetic Backgrounds

• Type: Article in International Scientific Journal
• Date: 2023

Authors

Rebelo, A (Author) Other; Duarte, B (Author) Other; R. Freitas, A (Author) Other; Peixe, L. (Author) FFUP; Antunes, Patrícia (Author) FCNAUP; Novais, Carla (Author) FFUP

Journal

Title: Microorganisms

Vol. 11 No. 9

Initial page: 2330

Publisher: MDPI

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 0 Citations

Scientific classification

CORDIS: Health sciences

FOS: Medical and Health sciences

Associated Projects

AGRIFOOD-XXI - AGRIFOOD-XXI

Other information

Authenticus ID: P-00Z-46G

DOI: 10.3390/microorganisms11092330

Abstract (EN): <jats:p>Acid stress poses a common challenge for bacteria in diverse environments by the presence of inorganic (e.g., mammals¿ stomach) or organic acids (e.g., feed additives; acid-based disinfectants). Limited knowledge exists regarding acid-tolerant strains of specific serotypes, clonal lineages, or sources in human/animal pathogens, namely non-typhoidal Salmonella enterica (NTS) and Enterococcus faecium (Efm). This study evaluated acidic pH and peracetic acid (PAA) susceptibility of Efm (n=72) and NTS (n=60) from diverse epidemiological/genetic backgrounds, and with multiple antibiotic resistance profiles. Efm minimum growth/survival-pH was 4,5-5/3-4 and for NTS 4,0-4,5/3,5-4,0. Among Efm, only those of clade-non-A1 (non-hospital associated) or food chain demonstrated greater tolerance to acidic pH compared to clade-A1 (hospital-associated clones) or clinical isolates (P<0.05). MDR (multidrug-resistant) NTS survived better to acidic pH (P<0.05). The MICPAA/MBCPAA was 70-120/80-150mg/L for Efm and 50-70/60-100mg/L for NTS. Efm-clade-A1 or MDR strains exhibited higher PAA tolerance than clade-non-A1 or non-MDR ones (P<0.05). Higher tolerance was found in non-MDR and clinical NTS than in food chain isolates (P<0.05), but not between different serogroups. This unique study identifies specific NTS or Efm populations more tolerant to acidic pH or PAA, emphasizing the need for further research to tailor control measures of public health and food safety within a One Health framework.</jats:p>

Language: English

Type (Professor's evaluation): Scientific