Influence of Serial Repitching on Beer Polypeptide Profiles

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Elsa Vieira (Author) Other; Cristina Moura (Author) Other; Tiago Almeida (Author) Other; Sonia Meireles (Author) Other; Tiago Brandao (Author) Other; Olivia Pinho (Author) FCNAUP; Isabel M P L V O Ferreira (Author) FFUP

Journal

Title: Journal of the American Society of Brewing Chemists

Vol. 70 No. 4

Pages: 275-279

ISSN: 0361-0470

Publisher: American Society of Brewing Chemists

Indexing

ISI Web of Knowledge - 10 Citations

ISI Web of Science

Scopus - 9 Citations

Scientific classification

FOS: Agrarian Sciences > Other Agrarian Sciences

CORDIS: Health sciences

Other information

Authenticus ID: P-002-EHD

DOI: 10.1094/asbcj-2012-0918-01

Abstract (EN): J. Am. Soc. Brew. Chem. 70(4):275-279, 2012 The evolution of polypeptide profiles during three successive fermentations with yeast generation N0, N+1, and N+2 was examined under large scale brewing conditions using 1,000 and 3,000 hL cylindroconical vessels, coded as G and H, respectively. ANOVA analyses indicated that the total protein content decreased significantly from wort (before yeast pitching) to green beer (samples collected after 2, 4, 6, 8, and 10 days of fermentation), and in general increased from green beer to mature beer (collected during beer conditioning after 12 and 14 days of yeast addition). Polypeptides from wort, green beer, and mature beer (without further treatments) were separated by chromatography according to their molecular size and by RP-HPLC. Quantitative differences were observed in peak areas, indicating the polypeptide, peptide, and amino acid changes throughout the fermentation and conditioning processes due to foam formation, protein precipitation, and yeast metabolism. A MANOVA was performed using RP-HPLC profile of beer from N0, N+1, and N+2 yeast generations produced in G and H fermentation vessels to establish the effect of vessel size and yeast generation. F values indicated greater importance of the yeast generation than the vessel size on the beer polypeptide profile. Yeast generation effect was notable in generation NO. Beer produced with generations N+1 and N+2 in the two cylindroconical vessels gave similar polypeptide profiles.

Language: English

Type (Professor's evaluation): Scientific

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No. of pages: 5