INFLUENCE OF DIFFERENT EXTRACTION CONDITIONS AND SAMPLE PRETREATMENTS ON QUANTIFICATION OF NITRATE AND NITRITE IN SPINACH AND LETTUCE

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Pinto, Edgar (Author) FCNAUP; Petisca, Catarina (Author) FCNAUP; Amaro, Luis (Author) Other; Pinho, Olívia (Author) FCNAUP; Ferreira, Isabel (Author) FCNAUP

Journal

Title: Journal of Liquid Chromatography and Related Technologies

Vol. 33 No. 5

Pages: 591-602

ISSN: 1082-6076

Publisher: Taylor & Francis

Indexing

ISI Web of Knowledge - 6 Citations

ISI Web of Science

Scopus - 10 Citations

Scientific classification

FOS: Medical and Health sciences > Health sciences

CORDIS: Health sciences

Other information

Authenticus ID: P-003-BP9

DOI: 10.1080/10826071003608306

Resumo (PT): Different sample pretreatment and extraction techniques are often used for analysis of nitrates and nitrites, however, the effects of these variables have not been properly examined. Comparative investigations were carried out with the objective of finding the most suitable conditions for quantification of nitrate and nitrite in spinach and lettuce. A rapid and cost effective RP-HPLC/UV method was validated and used to select the most appropriate extraction procedure to eliminate chromatographic interferences and to evaluate the influence of different sample pretreatments on the accuracy and reproducibility of the results obtained. Similar nitrate concentrations were obtained for fresh and two weeks frozen samples. Freeze drying and oven drying pretreatment of the spinach and lettuce material was inappropriate. No nitrite was detected in either fresh, freeze dried, oven dried, and frozen spinach or lettuce. <br> <br> Keywords: lettuce; nitrate; nitrite; RP-HPLC/UV; spinach <br> <a target="_blank" href="http://www.informaworld.com/smpp/content~db=all~content=a919596269~frm=titlelink "> Texto integral </a> <br> <br>

Abstract (EN): Different sample pretreatment and extraction techniques are often used for analysis of nitrates and nitrites, however, the effects of these variables have not been properly examined. Comparative investigations were carried out with the objective of finding the most suitable conditions for quantification of nitrate and nitrite in spinach and lettuce. A rapid and cost effective RP-HPLC/UV method was validated and used to select the most appropriate extraction procedure to eliminate chromatographic interferences and to evaluate the influence of different sample pretreatments on the accuracy and reproducibility of the results obtained. Similar nitrate concentrations were obtained for fresh and two weeks frozen samples. Freeze drying and oven drying pretreatment of the spinach and lettuce material was inappropriate. No nitrite was detected in either fresh, freeze dried, oven dried, and frozen spinach or lettuce.

Language: English

Type (Professor's evaluation): Scientific

Notes: <a href="http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=WOS&DestLinkTy pe=FullRecord&KeyUT=000275112200001">Indexado na ISI Web of Science</a>

No. of pages: 12