Nsaids as a drug repurposing strategy for biofilm control

• Type: Article in International Scientific Journal
• Date: 2020

Authors

Leão, C (Author) Other; Anabela Borges (Author) FEUP; Simões M (Author) FEUP

Journal

Title: Antibiotics

Vol. 9 No. 591

Pages: 1-19

Publisher: MDPI

Indexing

ISI Web of Knowledge - 0 Citations

ISI Web of Science

Scopus - 2 Citations

Associated Projects

Biocide_for_Biofilm - Desenvolvimento de novas soluções biocidas para o controlo efetivo de biofilmes

ABFISH - Desenvolvimento de novos antibióticos para aquacultura

Other information

Authenticus ID: P-00S-QSF

DOI: 10.3390/antibiotics9090591

Abstract (EN): Persistent infections, usually associated with biofilm-producing bacteria, are challenging for both medical and scientific communities. The potential interest in drug repurposing for biofilm control is growing due to both disinvestment in antibiotic R&D and reduced efficacy of the available panel of antibiotics. In the present study, the antibacterial and antibiofilm activities of four non-steroidal anti-inflammatory drugs (NSAIDs), piroxicam (PXC), diclofenac sodium (DCF), acetylsalicylic acid (ASA) and naproxen sodium (NPX) were evaluated against Escherichia coli and Staphylococcus aureus. The minimum inhibitory/bactericidal concentrations (MICs and MBCs) and the dose¿response curves from exposure to the selected NSAIDs were determined. MICs were found for PXC (800 µg/mL) and ASA (1750 µg/mL) against E. coli, and for DCF (2000 µg/mL) and ASA (2000 µg/mL) against S. aureus. No MBCs were found (>2000 µg/mL). The potential of NSAIDs to eradicate preformed biofilms was characterized in terms of biofilm mass, metabolic activity and cell culturability. Additionally, the NSAIDs were tested in combination with kanamycin (KAN) and tetracycline (TET). ASA, DCF and PXC promoted significant reductions in metabolic activity and culturability. However, only PXC promoted biofilm mass removal. Additive interactions were obtained for most of the combinations between NSAIDs and KAN or TET. In general, NSAIDs appear to be a promising strategy to control biofilms as they demonstrated to be more effective than conventional antibiotics. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Language: English

Type (Professor's evaluation): Scientific