Sphaerocyclamide, a prenylated cyanobactin from the cyanobacterium Sphaerospermopsis sp LEGE 00249

• Type: Article in International Scientific Journal
• Date: 2018

Authors

Martins, J (Author) Other; Leikoski, N (Author) Other; Wahlsten, M (Author) Other; Azevedo, J (Author) Other; Antunes, J (Author) Other; Jokela, J (Author) Other; Sivonen, K (Author) Other; Vitor Vasconcelos (Author) FCUP; Fewer, DP (Author) Other; Pedro N Leao (Author) Other

Journal

Title: Scientific Reports

Vol. 8

ISSN: 2045-2322

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 17 Citations

Scopus - 22 Citations

Other information

Authenticus ID: P-00P-PEY

DOI: 10.1038/s41598-018-32618-5

Abstract (EN): Cyanobactins are a family of linear and cyclic peptides produced through the post-translational modification of short precursor peptides. A mass spectrometry-based screening of potential cyanobactin producers led to the discovery of a new prenylated member of this family of compounds, sphaerocyclamide (1), from Sphaerospermopsis sp. LEGE 00249. The sphaerocyclamide biosynthetic gene cluster (sph) encoding the novel macrocyclic prenylated cyanobactin, was sequenced. Heterologous expression of the sph gene cluster in Escherichia coli confirmed the connection between genomic and mass spectrometric data. Unambiguous establishment of the orientation and site of prenylation required the full structural elucidation of 1 using Nuclear Magnetic Resonance (NMR), which demonstrated that a forward prenylation occurred on the tyrosine residue. Compound 1 was tested in pharmacologically or ecologically relevant biological assays and revealed moderate antimicrobial activity towards the fouling bacterium Halomonas aquamarina CECT 5000.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9