SAM-Based Immunosensor for the Analysis of Thyroxine (T4)

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Couto, RAS (Author) Other; Goncalves, LM (Author) Other; Goes, MS (Author) Other; Rodrigues, CMP (Author) Other; M. Beatriz Q. Garcia (Author) FFUP; Rodrigues, JA (Author) FCUP

Journal

Title: Journal of the Electrochemical Society

Vol. 164 No. 2

Pages: B103-B106

ISSN: 0013-4651

Publisher: Electrochemical Society, Inc.

Indexing

ISI Web of Knowledge - 13 Citations

Scopus - 14 Citations

Other information

Authenticus ID: P-00N-NH0

DOI: 10.1149/2.0561704jes

Abstract (EN): In this work T4, also known as thyroxine, a thyroid hormone with many relevant roles in human metabolism, was determined with an antibody-based biosensor, consisting on a multi thiol self-assembled monolayer (SAM) whereas a selective antibody was attached. Electroanalytical measurements were performed by impedance on a ferricyanide redox probe. A limit of detection of 1.5 ng mL(-1) and limit of quantification (LOQ) of 4.0 ngmL(-1) were obtained. This biosensor is a proof-of-concept for the creation of point-of-care electrochemical approach for the quantification of T4.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4