Development of an HPLC-UV method for determination of taurine in infant formulae and breast milk

• Type: Article in International Scientific Journal
• Date: 1997

Authors

Ferreira, IMPLVO (Author) FFUP; Nunes, MV (Author) Other; Mendes, E (Author) Other; Remiao, F (Author) FFUP; Ferreira, MA (Author) Other

Journal

Title: Journal of Liquid Chromatography and Related Technologies

Vol. 20

Pages: 1269-1278

ISSN: 1082-6076

Publisher: Taylor & Francis

Indexing

ISI Web of Knowledge - 15 Citations

Scopus - 22 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-001-CP8

DOI: 10.1080/10826079708010975

Abstract (EN): A rapid and accurate high performance liquid chromatographic (HPLC) procedure was proposed for routine and selective determination of taurine in infant formulas and breast milk. The sample preparation was simple, involving protein removal and filtration operations. Afterwards, taurine,was derivatised with o-phthalaldehyde/2-mercaptoethanol prior to injection onto a reversed phase column C-18 (S10ODS2). Isocratic elution was carried out using 0.05 M phosphate buffer pH 5.3/methanol (60:40) mixture. The effluent was monitored by a UV detector at 350 nm. A linear relationship was found between peak area and a concentration range of 1-70 mu g/mL. The detection limit was 0.3 mu g/mL. Effective separation and quantification was achieved in under six: minutes. No interference of other amino acids was observed.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10