Identification and expression studies of a catalase and a bifunctional catalase-peroxidase in Frankia strain R43

• Type: Article in International Scientific Journal
• Date: 2003

Authors

Tavares, F (Author) FCUP; Bernardo, L (Author) Other; Sellstedt, A (Author) Other

Journal

Title: Plant and Soil

Vol. 254

Pages: 75-81

ISSN: 0032-079X

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 5 Citations

Scopus - 4 Citations

Scientific classification

FOS: Agrarian Sciences > Agriculture, Forestry, and Fisheries

Other information

Authenticus ID: P-000-G5J

DOI: 10.1023/a:1024975618685

Abstract (EN): A monofunctional catalase and a bifunctional catalase-peroxidase were revealed by activity staining of nondenaturing PAGE in Frankia strain R43. Both enzymes were shown to be cytoplasmatic, growth regulated and expressed mainly during the stationary growth phase. Nevertheless, low levels of constitutive expression could also be detected during the early stages of growth. Immunoblot analyses using a polyclonal antibody raised against a catalase-peroxidase purified from Streptomyces reticuli showed a band of 83.2 kDa, with the same growth dependent pattern as obtained by the non-denaturing PAGE analyses. Induction studies revealed that both enzymes were strongly induced by raising the intracellular concentration of H2O2 with paraquat, but not with exogenous H2O2. In addition, no acquisition of tolerance to exogenous H2O2 was observed whatever the pretreatment of the inocula, i.e. despite the expression level of both hydroperoxidases.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7