Regeneration of the actinorhizal plant Myrica gale L. from epicotyl explants

• Type: Article in International Scientific Journal
• Date: 1998

Authors

Tavares, F (Author) FCUP; Abreu, I (Author) FCUP; Salema, R (Author) Other

Journal

Title: Plant Science

Vol. 135

Pages: 203-210

ISSN: 0168-9452

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 3 Citations

Scopus - 2 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-001-7FW

DOI: 10.1016/s0168-9452(98)00070-3

Abstract (EN): Induction and proliferation of axillary buds on epicotyl explants of Myrica gale was obtained on a medium consisting of Murashige and Skoog (MS) salts, vitamins of Gamborg et al., and supplemented with 2.5 mu M of 6-benzylaminopurine (BAP). No proliferation was obtained with the cytokinins 6-furfurylaminopurine (kinetin) or 6(gamma(2)-dimethplallylamino)-purine (2iP) at the tested concentrations. On the resulting microshoots, root induction and elongation were promoted by reduced medium strength (1/3 MS) and high light intensity (25-30 mu mol m(-2) s(-1)), respectively. The effects of indole-3-butyric acid (IBA) were more complex and dependent on medium strength and light intensity. However, a high rooting rate (73.3%) was obtained with 1.23 mu M IBA in 1/3 MS medium, under high light intensity. Rooted microshoots were successfully acclimatised under semi-aseptic conditions by transfer to a liquid medium, then to vermiculite and finally to soil.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8