Gas chromatography-ion trap mass spectrometry method for the simultaneous measurement of MDMA (ecstasy) and its metabolites, MDA, HMA, and HMMA in plasma and urine

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Daniel Gomes da Silva (Author) Other; de Pinho, PG (Author) FFUP; Helena Pontes (Author) Other; Luisa Ferreira (Author) Other; Paula Branco (Author) Other; Fernando Remiao (Author) FFUP; Felix Carvalho (Author) FFUP; Lurdes L Bastos (Author) FFUP; Helena Carmo (Author) FFUP

Journal

Title: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Vol. 878

Pages: 815-822

ISSN: 1570-0232

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 12 Citations

Scopus - 19 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-003-89M

DOI: 10.1016/j.jchromb.2010.01.042

Abstract (EN): The investigation of 3,4-methylenedioxymethamphetamine (MDMA: ecstasy) abuse requires very robust methods with high sensitivity and wide linearity ranges for the quantification of this drug of abuse and its main metabolites in body fluids. An optimized gas chromatography-ion trap mass spectrometry (GC-IT/MS) methodology with electron impact ionization addressing these issues is presented. The sample preparation involves an enzymatic hydrolysis of urine and plasma for conjugate cleavage, a SPE extraction, and a derivatization process. The method was fully validated in rat plasma and urine. Linearity for a wide concentration range was achieved for MDMA, and the metabolites 3,4-methylenedioxyamphetamine (MDA), 4-hydroxy-3-methoxyamphetamine (HMA) and 4-hydroxy-3-methoxymethamphetamine (HMMA). Limits of quantification were 2 ng/mL in plasma and 3.5 ng/mL in urine using a Selected Ion Monitoring detection mode. Selectivity, accuracy, precision, and recovery met the required criteria for the method validation. This GC-IT/MS method provides high sensitivity and adequate performance characteristics for the simultaneous quantification of MDMA, MDA, HMA and HMMA in the studied matrices.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8