ROLE OF TYPE-A AND TYPE-B MONOAMINE-OXIDASE ON THE FORMATION OF 3,4-DIHYDROXYPHENYLACETIC ACID (DOPAC) IN TISSUES FROM THE BRAIN OF THE RAT

• Type: Article in International Scientific Journal
• Date: 1990

Authors

GARRETT, MC (Author) FMUP; SOARESDASILVA, P (Author) Other

Journal

Title: Neuropharmacology

Vol. 29

Pages: 875-879

ISSN: 0028-3908

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 32 Citations

Scopus - 23 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-001-RGP

DOI: 10.1016/0028-3908(90)90136-f

Abstract (EN): The role of monamme oxidase (MAO), type A and B, on the deamination of dopamine in the striatum, nucleus accumbens and frontal cortex of the rat was studied. Levels of dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) tissue were quantified by means of high pressure liquid chromatography with electrochemical detection. Rats were given pargyline (75 mg/kg), selegyline (5 mg/kg) or clorgyline (2 mg/kg) by the intraperitoneal route, 60 min before sacrifice; in another set of experiments, clorgyline (2mg/kg, i.p.) was given 15 or 30 min before sacrifice. Only clorgyline and pargyline were found to reduce significantly the formation of DOPAC and HVA in all the three areas of brain under study (83-97% reduction). The inhibition of deamination of dopamine by clorgyline and pargyline was accompanied by an increase in levels of dopamine in tissue. The increase of the levels of amine in tissue, as a result of inhibition of MAOA was more marked in the frontal cortex (52% increase) and the accumbens (39% increase), than in the striatum (25% increase). The results suggest that a substantial amount of DOPAC in brain derives from the deamination of dopamine by MAOA. © 1990.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5