CALLI AND SUSPENSION-CULTURES FOR BIOMASS PRODUCTION OF EUPHORBIA-CHARACIAS L SUBSP CHARACIAS

• Type: Article in International Scientific Journal
• Date: 1989

Authors

FERNANDESFERREIRA, M (Author) Other; NOVAIS, JM (Author) Other; SALOME, M (Author) Other; PAIS, S (Author) Other

Journal

Title: Biotechnology Letters

Vol. 11

Pages: 259-264

ISSN: 0141-5492

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 7 Citations

Scopus - 8 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-001-SQ6

DOI: 10.1007/bf01031574

Abstract (EN): Friable calli and cell suspension cultures were obtained from leaf segments of Euphorbia characias L. subsp. characias, in Murashige and Skoog (MS) basal medium supplemented with 1g.l-1 casein hydrolyzate (CH), 5mg.l-1 ascorbic acid, 1.0mg.l-1 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.75mg.l-1 benzyl adenine (BA). The highest callus specific growth rate (¿=0.085.day-1), calculated for 1 year old calli cultures, was obtained with 0.25 mg.l-1 2,4-D and 0.50mg.l-1 BA. Suspension cultures started with an inoculum of 8.0×104 cells.ml-1 in supplemented liquid MS medium, gave a specific growth rate ¿=0.256.day-1. © 1989 Kluwer Academic Publishers.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6