Xanthone production in calli and suspended cells of Hypericum perforatum

• Type: Article in International Scientific Journal
• Date: 2001

Authors

Dias, ACP (Author) Other; Seabra, RM (Author) Other; Andrade, PB (Author) FFUP; Ferreres, F (Author) Other; Ferreira, MF (Author) Other

Journal

Title: Journal of Plant Physiology

Vol. 158

Pages: 821-827

ISSN: 0176-1617

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 33 Citations

Scopus - 33 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-000-V3H

DOI: 10.1078/0176-1617-00195

Abstract (EN): Calli and suspended cells of Hypericum perforatum accumulated xanthones with the 1,3,6,7 and 1,3,5,6-oxygenation pattern. Xanthone accumulation was highly influenced by hormone supplementation. Calli grown with 4.5 mu mol/L 2,4-D + 2.3 mu mol/L KIN had the highest specific xanthone production observed (0.73% DW). The use of BA instead of KIN reduced xanthone accumulation, resulting in the lowest production achieved (0.24 % DW). This effect was attenuated when both hormones were present. Calli displayed a positive linear relationship between NAA concentration, in the range of 4.5-22.5 mu mol/L, and total xanthone accumulation and the xanthone 1,3,6,7/1,3,5,6 ratio. However, the production of 1,3,5,6 xanthones was similar in ail NAA concentrations studied.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7