HPLC-UV-ESI-MS analysis of phenolic compounds and antioxidant properties of Hypericum undulatum shoot cultures and wild-growing plants

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Nuno Rainha (Author) Other; Kamila Koci (Author) Other; Ana Varela Coelho (Author) Other; Elisabete Lima (Author) Other; Jose Baptista (Author) Other; Manuel Fernandes Ferreira (Author) FCUP

Journal

Title: Phytochemistry

Vol. 86

Pages: 83-91

ISSN: 0031-9422

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 29 Citations

Scopus - 34 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-002-0S7

DOI: 10.1016/j.phytochem.2012.10.006

Abstract (EN): LC-UV and LC-MS analysis were used to study the phenolic composition of water extracts of Hypericum undulatum (HU) shoot cultures and wild-growing (WG) plants. Total phenolic content (TPC), determined using the Folin-Ciocalteu assay, and the antioxidant activity measured by two complementary methods were also performed for each sample. Mass spectrometry revealed several phenolics acids with quinic acid moieties, flavonols, mostly quercetin, luteolin and apigenin glycosides, flavan-3-ols (catechin and epicatechin) and the xanthonoid mangiferin. Differences in phenolic composition profile and TPC were found between the samples. The major phenolic in HU culture-growing (CG) samples is chlorogenic acid, followed by epicatechin, quercitrin and isoquercitrin. The WG plants presents hyperoside as the main phenolic, followed by isoquercitrin, chlorogenic acid and quercetin. The TPC and antioxidant activity were higher in samples from WG plants.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9