The vacuolar localization of a basic peroxidase isoenzyme responsible for the synthesis of alpha-3',4'-anhydrovinblastine in Catharanthus roseus (L) G. Don leaves

• Type: Article in International Scientific Journal
• Date: 1996

Authors

Sottomayor, M (Author) FCUP; dePinto, MC (Author) Other; Salema, R (Author) Other; DiCosmo, F (Author) Other; Pedreno, MA (Author) Other; Barcelo, AR (Author) Other

Journal

Title: Plant, Cell and Environment

Vol. 19

Pages: 761-767

ISSN: 0140-7791

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 29 Citations

Scopus - 41 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-001-E8B

DOI: 10.1111/j.1365-3040.1996.tb00412.x

Abstract (EN): Partially purified protein extracts of Catharanthus roseus leaves were able to couple catharanthine and vindoline to produce alpha-3',4'-anhydrovinblastine (AVLB) in a reaction strictly dependent on H2O2. This result, and the co-purification of peroxidase with AVLB synthetase activity, strongly suggest a peroxidase-like nature for the coupling enzyme, Only one peroxidase isoenzyme was detected in C. roseus leaves, and it was shown that this isoenzyme consists of a molecularly-heterogeneous basic peroxidase (EC 1 . 11 . 1 . 7) mainly located in the vacuole, These results suggest that a basic peroxidase located in the vacuole may be the main enzyme responsible for AVLB synthesis in C. roseus leaves, This isoenzyme was also found in cell walls where a peroxidase inhibitor was detected.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7