Genotoxic effects of binary mixtures of xenoandrogens (tributyltin, triphenyltin) and a xenoestrogen (ethinylestradiol) in a partial life-cycle test with Zebrafish (Danio rerio)

• Type: Article in International Scientific Journal
• Date: 2007

Authors

micael, j (Author) Other; reis-henriques, ma (Author) ICBAS; carvalho, ap (Author) FCUP; santos, mm (Author) Other

Journal

Title: Environment International

Vol. 33

Pages: 1035-1039

ISSN: 0160-4120

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 42 Citations

Scopus - 49 Citations

Scientific classification

FOS: Natural sciences > Earth and related Environmental sciences

Other information

Authenticus ID: P-004-6FG

DOI: 10.1016/j.envint.2007.06.004

Abstract (EN): A partial life-cycle test with the model fish Danio, rerio was performed in order to evaluate the genotoxic potential of binary mixtures of xenoandrogenic (tributyltin - TBT; triphenyltin - TPT) and an estrogenic compound (ethinylestradiol - EE2). Five days post-fertilisation larvae were diet-exposed to environmental relevant concentrations of TBT and TPT (25 ng/g- 100 ng/g), and water-exposed to ethinylestradiol (3.5 ng/L) for a four-month period; binary mixtures of TBT plus EE2 and TPT plus EE2 were run in parallel. The erythrocytic nuclear abnormalities (ENA) assay in circulating erythrocytes was used to evaluate genotoxicity in the end of the four-month exposure period. A significant increase (p<0.05, Kruskall-Wallis non-parametric ANOVA) in ENA frequency, in comparison with control animals, was observed in those animals exposed to TBT and TPT (the highest doses only), and to EE2 and the binary mixtures, although neither synergistic nor additive effects of the tested compounds were evident. Overall, the results clearly indicate that chronic exposure to low levels of TBT, TPT, EE2 and binary mixtures of TBT plus EE2 and TPT plus EE2 are genotoxic to zebrafish, which may suggest that wild fish populations may be under increased DNA damage in areas contaminated by these endocrine disrupting chemicals.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5