Evidence for a specific intracellular localization of an antisense oligonucleotide in K562 cells

• Type: Article in International Scientific Journal
• Date: 2005

Authors

Vasconcelos, MH (Author) FFUP; Maia, LF (Author) Other; Sousa, C (Author) Other; Beleza, SS (Author) Other; Guimaraes, JE (Author) FMUP

Journal

Title: Journal of Pharmacological Sciences

Vol. 99

Pages: 105-108

ISSN: 1347-8613

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 0 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-000-1N7

DOI: 10.1254/jphs.sce05001x

Abstract (EN): In order to be active, antisense oligonucleotides (ASOs) should be delivered to the nuclei of cells. The lack of effect of some ASOs might be explained by poor distribution inside the cell. Here we describe the study of the intracellular distribution of an ASO in a leukemic cell line in which the ASO was not showing an effect. We used fluorescein isothiocyanate-labeled ASO and fluorescent or confocal microscopy. The internalised ASO was localized in a specific intracellular juxtanuclear region, showing no cytoplasmic or nuclear diffusion. Transfection of the ASO improved cellular distribution to the cytoplasm and nuclei and improved the ASO effect.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4