Expression of arabinogalactan protein genes in pollen tubes of Arabidopsis thaliana

• Type: Article in International Scientific Journal
• Date: 2006

Authors

Pereira, LG (Author) FCUP; Coimbra, S (Author) FCUP; Oliveira, H (Author) Other; Monteiro, L (Author) Other; Sottomayor, M (Author) FCUP

Journal

Title: Planta

Vol. 223

Pages: 374-380

ISSN: 0032-0935

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 51 Citations

Scopus - 56 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-004-QH0

DOI: 10.1007/s00425-005-0137-4

Abstract (EN): The expression of "classical" arabinogalactan protein genes in pollen tubes of Arabidopsis thaliana was characterized by RT-PCR. Transcripts of Agp6 and Agp11 were consistently found to be more abundant in pollen tubes and seem to be pollen-specific. Transcripts of other AGP genes were also detected in pollen but in lesser amounts and in a non-specific fashion. Two reference genes, ubiquitin-conjugating enzyme 9 and tubulin beta-4 chain, were evaluated and selected for gene expression studies in pollen. Expression characterization was complemented with immunolocalization studies using monoclonal antibodies specific to several glycosidic epitopes of AGPs. These studies were performed on in vitro germinated pollen tubes with the antibodies MAC207 and LM2. MAC207 produced labelling at the tip of the pollen tube, while LM2 produced a ring-like fluorescence around the emerging region of the tube, suggesting a role of AGPs during Arabidopsis pollen tube germination. To our knowledge, this is the first report establishing the presence of AGPs on Arabidopsis pollen tubes.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7