Restriction fragment alleles of the rabbit IGHG genes with reference to the rabbit IGHGCH2 or e locus polymorphism

• Type: Article in International Scientific Journal
• Date: 2002

Authors

Esteves, PJ (Author) Other; Alves, PC (Author) FCUP; Ferrand, N (Author) FCUP; van der Loo, W (Author) Other

Journal

Title: Animal Genetics

Vol. 33

Pages: 309-311

ISSN: 0268-9146

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 6 Citations

Scopus - 7 Citations

Scientific classification

FOS: Agrarian Sciences > Animal and Dairy science

Other information

Authenticus ID: P-000-NCW

DOI: 10.1046/j.1365-2052.2002.00877.x

Abstract (EN): Among domesticated mammals, rabbit (Oryctolagus cuniculus ) is the only species possessing not more than one subclass of immunoglobulin (IgG) antibodies. The rabbit IGHGCH2 or e locus presents two serologically defined alleles, the e14 and e15 allotypes, which are correlated with amino acid variation at the IgG CH2-CH3 interface. Genetic studies, while revealing the adaptive value of this polymorphism, have relied so far entirely upon allo-antisera. Here we show how these alleles can be distinguished by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods. The proposed PCR-RFLP approach allows the monitoring of IGHG locus diversity in rabbit.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 3