Changes in the IgE-reacting protein profiles of Acer negundo, Platanus x acerifolia and Quercus robur pollen in response to ozone treatment

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Helena Ribeiro (Author) Other; Laura Duque (Author) Other; Raquel Sousa (Author) Other; Ana Cruz (Author) Other; Carlos Gomes (Author) FCUP; Joaquim Esteves da Silva (Author) FCUP; Ilda Abreu (Author) Other

Journal

Title: International Journal of Environmental Health Research

Vol. 24

Pages: 515-527

ISSN: 0960-3123

Publisher: Taylor & Francis

Indexing

ISI Web of Knowledge - 17 Citations

Scopus - 19 Citations

Scientific classification

FOS: Medical and Health sciences > Health sciences

Other information

Authenticus ID: P-009-W4Z

DOI: 10.1080/09603123.2013.865716

Abstract (EN): This study aims to investigate the effects of O-3 in protein content and immunoglobulin E (IgE)-binding profiles of Acer negundo, Platanus x acerifolia and Quercus robur pollen. Pollen was exposed to O-3 in an environmental chamber, at half, equal and four times the limit value for the human health protection in Europe. Pollen total soluble protein was determined with Coomassie Protein Assay Reagent, and the antigenic and allergenic properties were investigated by SDS-PAGE and immunological techniques using patients' sera. O-3 exposure affected total soluble protein content and some protein species within the SDS-PAGE protein profiles. Most of the sera revealed increased IgE reactivity to proteins of A. negundo and Q. robur pollen exposed to the pollutant compared with the non-exposed one, while the opposite was observed in P. x acerifolia pollen. So, the modifications seem to be species dependent, but do not necessarily imply that increase allergenicity would occur in atopic individuals.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 13