Quantitative analysis of organophosphorus pesticides in freshwater using an optimized firefly luciferase-based coupled bioluminescent assay

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Marques, SM (Author) Other; Esteves da Silva, JCGE (Author) FCUP

Journal

Title: Luminescence

Vol. 29

ISSN: 1522-7235

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 6 Citations

Scopus - 5 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-009-CYV

Abstract (EN): In this paper, a coupled bioluminescent assay, relying on the coupling of the enzymes acetylcholinesterase, S-acetyl-coenzyme A synthetase and firefly luciferase, for the detection and quantitation of organophosphorus pesticides, is presented. Using malathion as a model organophosphorus pesticide, the assay was optimized through statistical experimental design methodology, namely Plackett-Burman and central composite designs. The optimized method requires only 20 mu L of sample. The linear range for the assay was 2.5-15 mu M of malathion, with limits of detection and quantitation of 1.5 and 5.0 mu M, respectively. This simple, fast and robust method allows samples to be analyzed at room temperature and without any pretreatment. Copyright (c) 2013 John Wiley & Sons, Ltd.

Language: English

Type (Professor's evaluation): Scientific