Study of the interaction of a marine fulvic acid with Cu(II) at low concentration by molecular fluorescence and chemometrics

• Type: Article in International Scientific Journal
• Date: 1996

Authors

daSilva, JCGE (Author) FCUP; Machado, AASC (Author) Other

Journal

Title: Anales de Quimica

Vol. 92

Pages: 306-311

ISSN: 1130-2283

Publisher: Real Sociedad Española de Química

Indexing

ISI Web of Knowledge - 2 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-001-EZA

Abstract (EN): The Cu(II) complexation properties of a dilute solution of a marine fulvic acid sample (mfua) (25 mg/L solution in 0.1 mol/L potassium nitrate at pH=6) were studied by molecular fluorescence spectroscopy. The emission and the synchronous fluorescence (SyF) modes were used to compare their advantages and limitations. In the treatment of results, spectral data were pre-processed by a self-modeling mixture analysis method (SIMPLISMA) to detect different variations (components) in the raw data and to extract the spectra and quenching profiles of these components. These profiles of the quenching provoked by the Cu(II) ion on the mfua fluorescence were analyzed by the Stern-Volmer method and by a non-linear least squares procedure to obtain the conditional stability constants (K'). The log(K') for the strongest binding sites obtained from the non-linear analysis was 4.52(8) and 5.0(1), respectively from the emission and SyF spectral data. The overall log(K') obtained from the Stern-Volmer analysis was 4.32(7) and 4.42(6), respectively.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6