Firefly Bioluminescence: A Mechanistic Approach of Luciferase Catalyzed Reactions

• Type: Another Publication in an International Scientific Journal
• Date: 2009

Authors

Simone M Marques (Author) Other; Joaquim Esteves G E da Silva (Author) FCUP

Journal

Title: IUBMB Life

Vol. 61

Pages: 6-17

ISSN: 1521-6543

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 166 Citations

Scopus - 168 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-003-QRT

DOI: 10.1002/iub.134

Abstract (EN): Luciferase is a general term for enzymes catalyzing visible light emission by living organisms (bioluminescence). The studies carried out with Photinus pyralis (firefly) luciferase allowed the discovery of the reaction leading to light production. It can be regarded as a two-step process: the first corresponds to the reaction of luciferase's substrate, luciferin (LH(2)), with ATP-Mg(2+) generating inorganic pyrophosphate and an intermediate luciferyl-adenylate (LH(2)-AMP); the second is the oxidation and decarboxylation of LH(2)-AMP to oxyluciferin, the light emitter, producing CO(2), AMP, and photons of yellow-green light (550-570 nm). In a dark reaction LH(2)-AMP is oxidized to dehydroluciferyl-adenylate (L-AMP). Luciferase also shows acyl-coenzyme A synthetase activity, which leads to the formation of dehydroluciferyl-coenzyme A (L-CoA), luciferyl-coenzyme A (LH(2)-CoA), and fatty acyl-CoAs. Moreover luciferase catalyzes the synthesis of dinucleoside polyphosphates from nucleosides with at least a 3'-phosphate chain plus an intact terminal pyrophosphate moiety. The LH(2) stereospecificity is a particular feature of the bioluminescent reaction where each isomer, D-LH(2) or L-LH(2), has a specific function. Practical applications of the luciferase system, either in its native form or with engineered proteins, encloses the analytical assay of metabolites like ATP and molecular biology studies with luc as a reporter gene, including the most recent and increasing field of bioimaging. (C) 2008 IUBMB

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12