Another glimpse over the salting-out assisted liquid-liquid extraction in acetonitrile/water mixtures

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Ines Maria Valente (Author) Other; Luis Moreira Goncalves (Author) FCUP; Jose Antonio Rodrigues (Author) FCUP

Journal

Title: Journal of Chromatography A

Vol. 1308

Pages: 58-62

ISSN: 0021-9673

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 93 Citations

Scopus - 96 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-006-798

DOI: 10.1016/j.chroma.2013.08.014

Abstract (EN): The use of the salting-out effect in analytical chemistry is very diverse and can be applied to increase the volatility of the analytes in headspace extractions, to cause the precipitation of proteins in biological samples or to improve the recoveries in liquid-liquid extractions. In the latter, the salting-out process can be used to create a phase separation between water-miscible organic solvents and water. Salting-out assisted liquid-liquid extraction (SALLE) is an advantageous sample preparation technique aiming HPLC-UV analysis when developing analytical methodologies. In fact, some new extraction methodologies like QuEChERS include the SALLE concept. This manuscript discusses another point of view over SALLE with particular emphasis over acetonitrile-water mixtures for HPLC-UV analysis; the influence of the salting-out agents, their concentration and the water-acetonitrile volume ratios were the studied parameters. alpha-dicarbonyl compounds and beer were used as test analytes and test samples, respectively. The influence of the studied parameters was characterized by the obtained phase separation volume ratio and the fraction of alpha-dicarbonyls extracted to the acetonitrile phase. Results allowed the distribution of salts within three groups according to the phase separation and their extractability: (1) chlorides and acetates, (2) carbonates and sulfates and (3) magnesium sulfate; of all tested salts, sodium chloride had the highest influence on the alpha-dicarbonyls fraction extracted.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5