Molinate quantification in environmental water by a glutathione-S-transferase based biosensor

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Túlio I. S. Oliveira (Author) Other; Marcela Oliveira (Author) Other; Subramanian Viswanathan (Author) Other; M. Fátima Barroso (Author) Other; Luísa Barreiros (Author) FEUP; Olga C. Nunes (Author) FEUP; José A. Rodrigues (Author) FCUP; Pedro de Lima Neto (Author) Other; Selma E. Mazzetto (Author) Other; Simone Morais (Author) Other; Cristina Delerue Matos (Author) Other

Journal

Title: Talanta

Vol. 106

Pages: 249-254

ISSN: 0039-9140

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 23 Citations

Scopus - 22 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Associated Projects

UI0511 - PROJECTO ESTRATÉGICO - UI 511 - 2013-2014

Other information

Authenticus ID: P-005-2G0

DOI: 10.1016/j.talanta.2012.10.074

Abstract (EN): A glutathione-S-transferase (GST) based biosensor was developed to quantify the thiocarbamate herbicide molinate in environmental water. The biosensor construction was based on GST immobilization onto a glassy carbon electrode via aminosilane-glutaraldehyde covalent attachment. The principle supporting the use of this biosensor consists of the GST inhibition process promoted by molinate. Differential pulse voltammetry was used to obtain a calibration curve for molinate concentration, ranging from 0.19 to 7.9 mg L-1 and presenting a detection limit of 0.064 mg L-1. The developed biosensor is stable, and reusable during 15 days. The GST-based biosensor was successfully applied to quantify molinate in rice paddy field floodwater samples. The results achieved with the developed biosensor were in accordance with those obtained by high performance liquid chromatography. The proposed device is suitable for screening environmental water analysis and, since no sample preparation is required, it can be used in situ and in real-time measurements.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6