Effect of temperature and short chain fructooligosaccharides supplementation on the hepatic oxidative status and immune response of turbot (Scophthalmus maximus)

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Ines Guerreiro (Author) Other; Amalia Perez Jimenez (Author) Other; Benjamin Costas (Author) Other; Aires Oliva Teles (Author) FCUP

Journal

Title: Fish and Shellfish Immunology

Vol. 40

Pages: 570-576

ISSN: 1050-4648

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 36 Citations

Scopus - 35 Citations

Scientific classification

FOS: Agrarian Sciences > Agriculture, Forestry, and Fisheries

Other information

Authenticus ID: P-009-XHJ

DOI: 10.1016/j.fsi.2014.08.017

Abstract (EN): In this study the effect of diet supplementation with different levels of short-chain fructooligosaccharides (scFOS) on the hepatic oxidative status, hematology and innate immune parameters was evaluated in turbot reared at 15 degrees C and 20 degrees C. Four practical diets containing half of the protein provided by plant ingredients and the other half by fish meal were supplemented with scFOS at 0%, 0.5%, 1.0% and 2.0% and fed to turbot juveniles for 9 weeks. Independently of the rearing temperature, diet with 1% scFOS increased the haematocrit (Ht) while 2% scFOS augmented the mean corpuscular haemoglobin concentration (MCHC). Mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), white blood cells (%) and lysozyme were higher in fish reared at 15 degrees C, whereas red blood cells and neutrophil numbers increased in fish reared at 20 degrees C. Catalase (CAT) and glutathione peroxidase (GPX) activities were affected by rearing temperature being lower in fish reared at 20 degrees C. Compared to the control diet, at 15 degrees C, turbot fed 0.5 or 1% scFOS presented lower activities of CAT and glutathione reductase (GR). At 20 degrees C turbot fed the 2% scFOS diet presented lower activities of CAT and GPX. Lipid peroxidation (LPO) and glucose 6-phosphate dehydrogenase (G6PDH) activity were not affected by temperature nor dietary prebiotic incorporation. Results of this study suggest scFOS has no effect on innate immunology or hematology. High temperature (20 degrees C) does not induce turbot oxidative stress, but the recommended dietary scFOS incorporation level for counteracting oxidative stress may differ with other rearing temperature.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7