A new autosomal STR multiplex for canine genotyping

• Type: Article in International Scientific Journal
• Date: 2008

Authors

van Asch, B (Author) Other; Alves, C (Author) Other; Pereira, F (Author) Other; Gusmao, L (Author) Other; Amorim, A (Author) FCUP

Journal

Title: Forensic Science International: Genetics Supplement Series

Vol. 1 No. 1

Pages: 628-629

ISSN: 1875-1768

Publisher: Elsevier

Indexing

ISI Web of Knowledge

Scopus - 3 Citations

Other information

Authenticus ID: P-007-N2K

DOI: 10.1016/j.fsigss.2007.10.043

Abstract (EN): Canine genotyping is demanded in a vast range of situations that more commonly include pedigree verification, parentage and forensic investigations, and population structure, genetic origin and diversity studies. We developed a PCR multiplex that performs consistently up to the standards required for forensic and population analysis. Primers were designed for co-amplification of 9 tetra-nucleotide STR loci in an optimized PCR reaction for fragment size detection in automated capillary electrophoresis. The system includes FH3210, FH3241, FH2004, FH2658, FH4012, REN214L11, FH2010, FH2361 loci (on chromosomes 2, 8, 11, 14, 15, 16, 24 and 33, respectively) and a newly described locus on chromosome 38. Forensic parameters were calculated based on genotyping results obtained in a case-study population. This PCR multiplex for canine genotyping presented robustness and reproducibility of results.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 2