Homologous STRs in the X and Y chromosomes: A contribution to understanding human sexual chromosomes evolution

• Type: Article in International Conference Proceedings Book
• Date: 2000

Authors

Gusmao, L (Author) FCUP; Sanchez Diz, P (Author) Other; Gonzalez Neira, A (Author) Other; Brion, M (Author) Other; Lareu, MV (Author) Other; Amorim, A (Author) FCUP; Carracedo, A (Author) Other

Conference proceedings International

Title: PROGRESS IN FORENSIC GENETICS 8

Pages: 64-66

18th Congress of the International-Society-for-Forensic-Haemogenetics (ISFH)

SAN FRANCISCO, CA, AUG 17-21, 1999

Indexing

ISI Web of Knowledge - 2 Citations

Scientific classification

FOS: Medical and Health sciences > Other medical sciences

Other information

Authenticus ID: P-008-KM5

Abstract (EN): DYS391 and DYS393 primers, described in the Genome Data Base, were used to amplify DNA samples from both male and female origin. After the amplification of DNA from male blood samples it was possible to observe additional amplification products to the normal alleles for both systems when an annealing temperature of 55 degrees C was used. In addition, in the same size range a clear polymorphism was detected in females for both systems. For DYS391, a first screening in males on these minor amplified products allowed the identification of 5 different alleles. After the amplification of male DNA samples only single band patterns were observed while using the same conditions to amplify female DNA it was possible to observe the same polymorphism but with the presence of heterozygous patterns, as expected for a diploid genetic system. In order to improve the applicability of this system in forensic genetics, an alternative primer was designed to amplify the Y-chromosome specific STR.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 3