Characterization of four short tandem repeat loci (THO1, VWA31/A, CD4, and TP53) in northern Portugal

• Type: Article in International Scientific Journal
• Date: 1997

Authors

Amorim, A (Author) FCUP; Gusmao, L (Author) Other; Prata, MJ (Author) FCUP; Silva, F (Author) Other; Bessa, I (Author) Other

Journal

Title: Human Biology

Vol. 69

Pages: 31-40

ISSN: 0018-7143

Publisher: Wayne State University Press

Indexing

ISI Web of Knowledge - 22 Citations

Scopus - 20 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-001-C0Z

Abstract (EN): Allele and genotype frequencies for two tetrameric and two pentameric short tandem repeat (STR) loci (THO1, VWA31/A, CD4, and TP53) were determined in a population sample from northern Portugal. Genotyping of PCR amplification products was done using polyacrylamide gel electrophoresis followed by silver staining; heteroduplex analysis was performed to distinguish THO1 genotypes involving allele 10 and the nonconsensus allele 9.3. For all loci allele frequencies fitted the distribution patterns generally observed in European populations, The observed genotype distributions do not deviate significantly from Hardy-Weinberg expectations, although for VWA31/A a significant excess of heterozygotes involving allele 17 was found. Mother-child pair analyses confirmed the regular Mendelian pattern of inheritance, Because the information content of these systems is high and because their genotyping is technically reliable and simple, CD4, THO1, VWA31/A, and TP53 are appropriate genetic systems for anthropological genetics.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10