Somatic and germinal mutations in STRs

• Type: Article in International Conference Proceedings Book
• Date: 2000

Authors

Amorim, A (Author) FCUP; Alves, C (Author) Other; Gusmao, L (Author) FCUP

Conference proceedings International

Title: PROGRESS IN FORENSIC GENETICS 8

Pages: 37-39

18th Congress of the International-Society-for-Forensic-Haemogenetics (ISFH)

SAN FRANCISCO, CA, AUG 17-21, 1999

Indexing

ISI Web of Knowledge - 0 Citations

Scientific classification

FOS: Medical and Health sciences > Other medical sciences

Other information

Authenticus ID: P-001-252

Abstract (EN): We report the study of meiotic and mitotic stability of 12 autosomal STRs included in Perkin Elmer's Profiler Plus Kit (D381358, D5S818, D7S820, D8S1179, D13S317, D18S51, D21S11, FGA, and VWA) and the Promega CTTv multiplex (which includes CSF1PO, TH01, TPO and VWA). PCR amplification and genotyping were performed according to manufacturer's instructions using ABI 377 or ABI 310 automatic sequencers. We searched for (a) transmission incompatibilities and (b) presence of extra alleles (more than two amplified fragments in the same individual). We have observed one exclusion in each of the systems: D3S1358, D7S820 and D8S1179. All are explainable by single step mutations. We have furthermore observed in D3S1358 a healthy individual with three PCR fragments both in blood and buccal swab DNA extracts.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 3