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Rapid identification of Aspergillus fumigatus within the section Fumigati

Title
Rapid identification of Aspergillus fumigatus within the section Fumigati
Type
Article in International Scientific Journal
Year
2011
Authors
Rita Serrano
(Author)
Other
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Leonor Gusmao
(Author)
Other
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Antonio Amorim
(Author)
FCUP
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Journal
Title: BMC MicrobiologyImported from Authenticus Search for Journal Publications
Vol. 11
Final page: 82
ISSN: 1471-2180
Publisher: Springer Nature
Scientific classification
FOS: Natural sciences > Biological sciences
Other information
Authenticus ID: P-002-SBQ
Abstract (EN): Background: New fungal species that are morphologically similar to Aspergillus fumigatus were recently described and included in section Fumigati. Misidentification of such fungal species, particularly of the human pathogens, Aspergillus lentulus, Neosartorya fischeri, Neosartorya hiratsukae, Neosartorya pseudofischeri and Neosartorya udagawae, has been increasingly reported by numerous clinical labs. Nevertheless, A. fumigatus still accounts for more than 90% of all invasive aspergillosis cases. The purpose of the present study was to develop a rapid method for the molecular identification of A. fumigatus to distinguish it from other species within the section Fumigati. Results: A multiplex PCR was developed using prior information based on beta-tubulin (beta tub) and rodlet A (rodA) partial gene sequences. PCR amplification of beta tub and rodA fragments resulted in a distinctive electrophoretic pattern in A. fumigatus and N. udagawae. The polymorphisms found in the smallest amplified sequence of beta tub (153 bp) and rodA (103 bp) genes were then compared among and within species of this taxonomic section. beta tub was able to differentiate among 13 individual species and two groups of species that included the pathogenic fungus A. lentulus. A more limited number of sequences were available for rodA; nevertheless, we were able to distinguish Aspergillus viridinutans, N. hiratsukae and N. udagawae. Conclusions: The assay described in the present study proved to be specific and highly reproducible, representing a fast and economic way of targeting molecular identification of the relevant mould, A. fumigatus, in clinical laboratories.
Language: English
Type (Professor's evaluation): Scientific
Contact: ricjparaujo@yahoo.com
No. of pages: 7
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