Toxicology of a Microcystis ichthyoblabe waterbloom from Lake Oued Mellah (Morocco)

• Type: Article in International Scientific Journal
• Date: 2002

Authors

Sabour, B (Author) Other; Loudiki, M (Author) Other; Oudra, B (Author) Other; Vasconcelos, V (Author) FCUP; Martins, R (Author) Other; Oubraim, S (Author) Other; Fawzi, B (Author) Other

Journal

Title: Environmental Toxicology

Vol. 17

Pages: 24-31

ISSN: 1520-4081

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 32 Citations

Scopus - 37 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-000-QD9

DOI: 10.1002/tox.10028

Abstract (EN): In the Lake Oued Mellah cyanobacteria waterblooms occur periodically in late spring and summer with Microcystis ichthyoblabe as the main bloom-forming species. In 1999, a heavy waterbloom of M. ichthyoblabe occurred during May June with a maximal biomass of 298 mg/l. During this period, several bloom samples were collected. The toxicity assessment was done by mouse and brine shrimp (Artemia) bioassays. Apart from the sample collected on 15/06/1999, all the other samples were toxic by mouse bioassay. The LD50 determined by intraperitoneal injection to mice during active cyanobacterial growth and decline phases were 518 and 1924 mgDW/kg respectively. Using Artemia bioassay, the 24hLC(50) varied from 6.0 to 40.6 mg/ml and the 48hLC(50) ranged from 2.8 to 18.2 mg/ml. The separation and identification of microcystin variants was performed by high performance liquid chromatography-photodiode array detection. Eleven toxins were separated and preliminarily identified as microcystin variants as they exhibit a typical UV spectra like the microcystin-LR standard. The quantification of total microcystins determined by enzyme-linked immunosorbent assay showed that the contents were varied between 0.1 and 0.76 mug/g DW (C) 2002 by Wiley Periodicals, Inc.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8