Peptide diversity in strains of the cyanobacterium Microcystis aeruginosa isolated from Portuguese water supplies

• Type: Article in International Scientific Journal
• Date: 2009

Authors

Joana Martins (Author) Other; Martin L Saker (Author) Other; Cristiana Moreira (Author) Other; Martin Welker (Author) Other; Jutta Fastner (Author) Other; Vitor M Vasconcelos (Author) FCUP

Journal

Title: Applied Microbiology and Biotechnology

Vol. 82

Pages: 951-961

ISSN: 0175-7598

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 38 Citations

Scopus - 40 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-003-KY1

DOI: 10.1007/s00253-009-1877-z

Abstract (EN): Strains of the cyanobacterium Microcystis aeruginosa were isolated into pure culture from a variety of lakes, rivers, and reservoirs in Portugal. Samples were tested with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) to investigate the presence of various peptide groups including aeruginosins, microginins, anabaenopeptins, cyanopeptilins, microcystins, and microviridins and other peptide-like compounds. Binary data, based on the presence and absence of different peptide groups, were analyzed by phylogenetic inference. DNA was also extracted from the samples and tested using a range of primers. Those strains that gave positive results for a Microcystis-specific primer pair were further analyzed for the presence of genes linked to the biosynthesis of microginin and microcystin. The results showed that a wide range of microcystin forms were produced by the strains among which MC-LR, -FR, -RR, -WR, and -YR were the most common. The peptide profiles obtained from the MALDI analysis were assessed using cluster analysis which resulted in the formation of distinct groups or chemotypes.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11