VIABILITY AND RESPIRATORY ACTIVITY OF PSEUDOMONAS-SYRINGAE CELLS STARVED IN BUFFER

• Type: Article in International Scientific Journal
• Date: 1995

Authors

cabral, jps (Author) FCUP

Journal

Title: Canadian Journal of Microbiology

Vol. 41

Pages: 372-377

ISSN: 0008-4166

Publisher: NRC Research Press

Indexing

ISI Web of Knowledge - 5 Citations

Scopus - 4 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-001-H2J

Abstract (EN): Pseudomonas syringae cells starved in buffer released orcinol-reactive molecules and materials that absorbed ultraviolet light. The number of cells culturable in nutrient medium decreased more rapidly than the number of intact particles determined by microscopy. The results suggested that starvation resulted in the lysis of an increasing number of cells, and that a fraction of the intact particles were not culturable. Starvation also resulted in a decrease in the rate of oxygen consumption with acetate, glycerol, and succinate, but at different levels. Whereas the respiration of acetate and glycerol decreased concomitantly with culturability, the respiration of succinate decreased to levels similar to the concentration of intact cells, suggesting that all intact particles respired the succinate, but only the culturable cells respired the acetate and glycerol. The results suggest that measuring the activity of the electron-transport system can overestimate the viability of starved bacterial cells, and that complex metabolic activities such as the respiration of acetate and glycerol are probably better suited for the evaluation of this parameter.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6