Synthesis of dehydroluciferin by firefly luciferase: Effect of dehydroluciferin, coenzyme A and nucleoside triphosphates on the luminescent reaction

• Type: Article in International Scientific Journal
• Date: 1997

Authors

fontes, r (Author) FMUP; dukhovich, a (Author) Other; sillero, a (Author) Other; sillero, mag (Author) Other

Journal

Title: Biochemical and Biophysical Research Communications

Vol. 237

Pages: 445-450

ISSN: 0006-291X

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 48 Citations

Scopus - 49 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-001-AWP

DOI: 10.1006/bbrc.1997.7161

Abstract (EN): The formation of dehydroluciferin (L) from luciferin (LH2) in the reaction catalyzed by firefly luciferase (EC 1.13.12.7) has been studied. The E.LH2-AMP complex may follow two different pathways: towards production of light and towards the synthesis of the E.L-AMP complex, This last step has an inhibitory effect on light emission as molecules of the enzyme are trapped in a light unproductive complex, The effects of CoA and nucleoside 5'-triphosphates (NTPs) on light emission are quantitatively different, CoA combines with the L moiety of the E.L-AMP complex, yielding L-CoA, promoting liberation of free luciferase, and increasing light yield, NTP reacts with the AMP moiety of the same complex, generating adenosine(5')tetraphospho(5')nucleoside (Ap(4)N) and, probably, the E.L complex and scarcely increasing light production, The results are discussed in relation to previous reports, by others, on luciferase. (C) 1997 Academic Press.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6