Chemical synthesis and firefly luciferase produced dehydroluciferyl-coenzyme A

• Type: Article in International Scientific Journal
• Date: 2004

Authors

Fraga, H (Author) Other; da Silva, JCGE (Author) FCUP; Fontes, R (Author) FMUP

Journal

Title: Tetrahedron Letters

Vol. 45

Pages: 2117-2120

ISSN: 0040-4039

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 12 Citations

Scopus - 12 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-000-BJ1

DOI: 10.1016/j.tetlet.2004.01.050

Abstract (EN): Dehydroluciferyl-coenzyme A (L-CoA) was chemically synthesized and characterized by MS, UV-vis spectrometry and RP-HPLC. The identity of the chemically synthesized compound with the one that was produced by firefly luciferase was confirmed. Moreover, the reversibility of the enzymatic conversion of dehydroluciferin reversible arrow dehydroluciferyl-adenylate reversible arrow L-CoA was also confirmed. The chemical synthesis of L-CoA, described here, may help the clarification of the activator effect of CoA on luciferase bioluminescent assays, in which the enzyme catalyzed formation of L-CoA and the consequent destruction of L-AMP is one of the possible explanations for that effect.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4