Deletion of the neuropeptide Y (NPY) Y-1 receptor gene reveals a regulatory role of NPY on catecholamine synthesis and secretion

• Type: Article in International Scientific Journal
• Date: 2006

Authors

cavadas, c (Author) Other; cefai, d (Author) Other; rosmaninho-salgado, j (Author) Other; vieira-coelho, ma (Author) FMUP; moura, e (Author) Other; busso, n (Author) Other; pedrazzini, t (Author) Other; grand, d (Author) Other; rotman, s (Author) Other; waeber, b (Author) Other; aubert, jf (Author) Other; grouzmann, e (Author) Other

Journal

Title: Proceedings of the National Academy of Sciences of the United States of America

Vol. 103

Pages: 10497-10502

ISSN: 0027-8424

Publisher: National Academy of Sciences

Indexing

ISI Web of Knowledge - 42 Citations

Scopus - 47 Citations

Other information

Authenticus ID: P-004-JN1

DOI: 10.1073/pnas.0600913103

Abstract (EN): The contribution of neuropeptide Y (NPY), deriving from adrenal medulla, to the adrenosympathetic tone is unknown. We found that in response to NPY, primary cultures of mouse adrenal chromaffin cells secreted catecholamine, and that this effect was abolished in cultures from NPY Y-1 receptor knockout mice (Y-1-/-). Compared with wild-type mice (Y-1+/+), the adrenal content and constitutive release of catecholamine were increased in chromaffin cells from Y-1-/- mice. In resting animals, catecholamine plasma concentrations were higher in Y-1-/- mice. Comparing the adrenal glands of both genotypes, no differences were observed in the area of the medulla, cortex, and X zone. The high turnover of adrenal catecholamine in Y-1-/- mice was explained by the enhancement of tyrosine hydroxylase (TH) activity, although no change in the affinity of the enzyme was observed. The molecular interaction between the Y-1 receptor and TH was demonstrated by the fact that NPY markedly inhibited the forskolin-induced luciferin activity in Y-1 receptor-expressing SK-N-MC cells transfected with a TH promoter sequence. We propose that NPY controls the release and synthesis of catecholamine from the adrenal medulla and consequently contributes to the sympathoadrenal tone.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6