Molecular insights into mitochondrial dysfunction in cancer-related muscle wasting

• Type: Article in International Scientific Journal
• Date: 2014

Authors

D. Antunes (Author) Other; A. I. Padrão (Author) Other; E. Maciel (Author) Other; D. Santinha (Author) Other; P. Oliveira (Author) Other; R. Vitorino (Author) Other; D. Moreira-Gonçalves (Author) Other; B. Colaço (Author) Other; M. J. Pires (Author) Other; C. Nunes (Author) Other; L. L. Santos (Author) Other; José Alberto Duarte (Author) Other; M. R. Domingues (Author) Other

Journal

Title: Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

Vol. 1841

Pages: 896-905

ISSN: 1388-1981

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 53 Citations

Scopus - 50 Citations

Scientific classification

FOS: Social sciences

Other information

Authenticus ID: P-009-C7N

DOI: 10.1016/j.bbalip.2014.03.004

Abstract (EN): Alterations in muscle mitochondrial bioenergetics during cancer cachexia were previously suggested; however, the underlying mechanisms are not known. So, the goal of this study was to evaluate mitochondrial phospholipid remodeling in cancer-related muscle wasting and its repercussions to respiratory chain activity and fiber susceptibility to apoptosis. An animal model of urothelial carcinoma induced by exposition to N-butyl-N-(4-hydroxybutyl)-nitrosamine (BBN) and characterized by significant body weight loss due to skeletal muscle mass decrease was used. Morphological evidences of muscle atrophy were associated to decreased respiratory chain activity and increased expression of mitochondrial UCP3, which altogether highlight the lower ability of wasted muscle to produce ATP. Lipidomic analysis of isolated mitochondria revealed a significant decrease of phosphatidic acid, phosphatidylglycerol and cardiolipin in BBN mitochondria, counteracted by increased phosphatidylcholine levels. Besides the impact on membrane fluidity, this phospholipid remodeling seems to justify, at least in part, the lower oxidative phosphorylation activity observed in mitochondria from wasted muscle and their increased susceptibility to apoptosis. Curiously, no evidences of lipid peroxidation were observed but proteins from BBN mitochondria, particularly the metabolic ones, seem more prone to carbonylation with the consequent implications in mitochondria functionality. Overall, data suggest that bladder cancer negatively impacts skeletal muscle activity specifically by affecting mitochondrial phospholipid dynamics and its interaction with proteins, ultimately leading to the dysfunction of this organelle. The regulation of phospholipid biosynthetic pathways might be seen as potential therapeutic targets for the management of cancer-related muscle wasting. © 2014 Published by Elsevier B.V.

Language: English

Type (Professor's evaluation): Scientific