Spatially distinct mitochondrial populations exhibit different mitofilin levels

• Type: Article in International Scientific Journal
• Date: 2012

Authors

ferreira, rmp (Author) Other; vitorino, r (Author) Other; padrao, ai (Author) Other; moreira-goncalves, d (Author) Other; alves, rmp (Author) Other; duarte, ja (Author) FADEUP; amado, f (Author) Other

Journal

Title: Cell Biochemistry and Function

Vol. 30

Pages: 395-399

ISSN: 0263-6484

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 7 Citations

Scopus - 7 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-002-8ES

DOI: 10.1002/cbf.2811

Abstract (EN): Subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria exhibit unique biochemical and functional properties; however, their association with structural membrane proteins that control mitochondrial morphology and functionality in striated muscle tissue was never reported. In IMF and SS mitochondria isolated from rat heart and gastrocnemius muscle, we analysed the expression levels of mitofilin, a mitochondria-associated protein involved in organelle structure maintenance. The statistically significant higher amounts of mitofilin detected in IMF compared with SS mitochondria, 37-fold in cardiac tissue and 3.8-fold in gastrocnemius, together with the specific energetic requirements of these mitochondrial populations highlight the importance of mitofilin in oxidative phosphorylation functionality and in mitochondrial plasticity in striated muscle. The differential expression levels of mitofilin between IMF and SS also suggest that this protein can be used as a specific molecular marker to comparatively discriminate spatially distant mitochondrial populations. Copyright (C) 2012 John Wiley & Sons, Ltd.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5